City got the better of rivals Hereford in a 1-0 victory at TigerTurf Stadium.
A hard-fought game in which Jordon Thompson broke the deadlock when he rose highest from a cross to head home.
A superb win made more significant by the fact that there were 2,826 fans to witness, which is the highest attendance since boxing day 1994.
City looked the more threatening team in the first 20 minutes but unable to create any clear-cut chances.
It was a very physical game with the Gloucester players standing up to the challenge. A combative first half performance dealing with the wind against, the Tigers went in at halftime with the score 0-0.
The second half got underway, and it was like the first half with City looking the more dominant team without threatening the Hereford goal.
In the 71st minute, Kevin Dawson curled in a teasing ball to the backstick where Thompson arrived late to find the back of the net with a header. A well-deserved goal after applying pressure throughout.
Matt green was subbed on for `Matt McClure after City had taken the lead.
Moments later, Ollie Hulbert was slid through on goal and with only the goalkeeper to beat his shot went narrowly wide.
The last guilt edge chance fell to Louis Hall when Hulbert cut the ball back to the edge of the box, but his low shot brought a good save out of the Hereford shot stopper.
Lucas Tomlinson made way for Tom Harrison in the 85th minute
The final bit of action saw the Gloucester Captain, Dawson, drive 70 yards with the ball into the final third and win a foul. This summed up not only his performance but the whole team.
City showed real grit and determination. The team looked organised and energetic from start to finish. Lee Mansell and his men will be looking to finish the season strong and build a platform to take into next season. Gloucester have got play-off chasing York City Saturday, which will no doubt be a tough fixture.