Tim Harris has confirmed that Sam Avery will be fit ahead of the trip to Weston-super-Mare on Tuesday evening.
There was some concern that Avery, 28, would not be ready to face the Seagulls tonight but the 2015/16 Player of the Season has shaken off a knock and will be available for selection.
The Tigers will be looking for their first win in the National League South, against a side who are yet to pick up a point from their opening three games.
Gloucester have one, hitting back to draw 2-2 with Wealdstone on Saturday thanks to goals from Keiran Thomas and Robbie Cundy.
“We are not firing at the moment, but I was pleased that we came back against Wealdstone,” Harris said.
“Once their second goal went in I was fearing we may buckle, but we found some resolve, character and desire to earn the point.
“We shot ourselves in the foot, but I felt they grew some inner strength and didn’t give up.
“It was important we got something out of the game and picked up that first point, but now we want that first win.”
Midfielder Joe Hanks is unavailable, while Karnell Chambers and Lewis Hall are out with hamstring injuries.
Chambers has a chance of returning for Saturday’s home match against Hemel Hempstead Town, while Hall is progressing well and is hoping to back within three weeks.
Chris Knowles is still out with a knee injury and likely faces another five weeks on the sideline.